Chicken Avidin Exhibits Pseudo-catalytic Properties
نویسندگان
چکیده
منابع مشابه
Chicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin.
Chicken avidin and bacterial streptavidin are proteins familiar from their use in various (strept)avidin-biotin technological applications. Avidin binds the vitamin biotin with the highest affinity known for non-covalent interactions found in nature. The gene encoding avidin (AVD) has homologues in chicken, named avidin-related genes (AVRs). In the present study we used the AVR genes to produce...
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A cDNA encoding for zebrafish gamma-glutamyl hydrolase (gammaGH) was cloned and inserted into a pET43.1a vector via SmaI and EcoRI sites and expressed in Rosetta (DE3) cells as a Nus-His-tag fusion enzyme (NH-zgammaGH). After induction with isopropyl thiogalactoside, the enzyme was purified with a Ni-Sepharose column, and approximately 8 mg of pure enzyme was obtained per liter of culture. The ...
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Protein phosphatase-1 (PP1) is expressed ubiquitously and is involved in many eukaryotic cellular functions, although PP1 enzyme activity could not be detected in the social amoeba Dictyostelium discoideum cell extracts. In the present paper, we show that D. discoideum has a single copy gene that codes for the catalytic subunit of PP1 (DdPP1c). DdPP1c is expressed throughout the D. discoideum l...
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ADAMs are membrane-anchored glycoproteins with functions in fertilization, heart development, neurogenesis, and protein ectodomain shedding. Here we report an evaluation of the catalytic activity of recombinantly expressed soluble forms of ADAM19, a protein that is essential for cardiovascular morphogenesis. Proteolytic activity of soluble forms of ADAM19 was first demonstrated by their autocat...
متن کاملTesting of Catalytic Properties
1. Definition of Catalytic Properties 1.1. Conversion 1.2. Selectivity 1.3. Yield 1.4. Catalyst Deactivation and Sustainability 2. Reactor Types 2.1. Batch Reactor 2.2. Perfectly Mixed Flow Reactor 2.3. Plug Flow Reactor 3. Intrinsic Kinetics 3.1. External Mass and Heat Transport Limitations 3.2. Internal Mass and Heat Transport Limitations 3.3. Flow Pattern Ideality 4. Specific Reactor Types 4...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2001
ISSN: 0021-9258
DOI: 10.1074/jbc.m102018200